Controlling gene expression in plants using synthetic zinc finger transcription factors
نویسندگان
چکیده
منابع مشابه
Controlling gene expression in plants using synthetic zinc finger transcription factors.
Synthetic zinc finger proteins can be fused to transcriptional regulatory domains to create artificial transcription factors that modulate the expression of a specific target gene. Recent studies have demonstrated that synthetic zinc finger domains can be constructed to bind DNA sequences with a high degree of specificity. To devise a general strategy for controlling plant gene expression with ...
متن کاملHeritable endogenous gene regulation in plants with designed polydactyl zinc finger transcription factors.
Zinc finger transcription factors (TFs(ZF)) were designed and applied to transgene and endogenous gene regulation in stably transformed plants. The target of the TFs(ZF) is the Arabidopsis gene APETALA3 (AP3), which encodes a transcription factor that determines floral organ identity. A zinc finger protein (ZFP) was designed to specifically bind to a region upstream of AP3. AP3 transcription wa...
متن کاملRegulation of transgene expression in plants with polydactyl zinc finger transcription factors.
Designer zinc finger transcription factors (TFs(ZF)) have been developed to control the expression of transgenes and endogenous genes in mammalian cells. Application of TFs(ZF) technology in plants would enable a wide range of both basic and applied studies. In this paper, we report the use of TFs(ZF) to target a defined 18-bp DNA sequence to control gene expression in plant cells and in transg...
متن کاملAdaptive Evolution in Zinc Finger Transcription Factors
The majority of human genes are conserved among mammals, but some gene families have undergone extensive expansion in particular lineages. Here, we present an evolutionary analysis of one such gene family, the poly-zinc-finger (poly-ZF) genes. The human genome encodes approximately 700 members of the poly-ZF family of putative transcriptional repressors, many of which have associated KRAB, SCAN...
متن کاملChemically regulated zinc finger transcription factors.
Ligand-dependent transcriptional regulators were generated by fusion of designed Cys(2)-His(2) zinc finger proteins and steroid hormone receptor ligand binding domains. To produce novel DNA binding domains, three-finger proteins binding specific 9-base pair sequences were constructed from modular building blocks. Fusion of these zinc finger proteins to a transcriptional activation domain and to...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Plant Journal
سال: 2002
ISSN: 0960-7412
DOI: 10.1046/j.1365-313x.2002.01492.x